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| Opening Round: Tuesday, March 16, 2010 | |
| UD Arena Dayton, Ohio Host: University of Dayton | |
| First and Second Rounds: Thursday and Saturday, March 18 and 20, 2010 | |
| New Orleans Arena New Orleans, Louisiana Host: Tulane University |
Dunkin Donuts Center Providence, Rhode Island Host: Big East Conference/Providence College |
| HP Pavilion San Jose, California Host: San Jose State University |
Ford Center Oklahoma City, Oklahoma Host: Big 12 Conference |
| First and Second Rounds: Friday and Sunday, March 19 and 21, 2010 | |
| HSBC Arena Buffalo, New York Host: MAAC/Canisius/Niagara |
Jacksonville Veterans Memorial Arena Jacksonville, Florida Host: Jacksonville University |
| Bradley Center Milwaukee, Wisconsin Host: Marquette University |
Spokane Memorial Arena Spokane, Washington Host: Washington State University |
| Regionals: Thursday and Saturday, March 25 and 27, 2010 | |
| East Regional Carrier Dome Syracuse, New York Host: Syracuse University |
West Regional Energy Solutions Arena Salt Lake City, Utah Host: University of Utah |
| Regionals: Friday and Sunday, March 26 and 28, 2010 | |
| Midwest Regional Edward Jones Dome St. Louis, Missouri Host: Missouri Valley Conference |
South Regional Reliant Stadium Houston, Texas Host: University of Houston |
| Final Four: April 3 and 5, 2010 | |
|
Lucas Oil Stadium Indianapolis, Indiana Host: Butler University/Horizon League | |
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