Blackburn gave up a hit in the first inning, then walked the bases loaded in the second before Twins trainer Dave Pruemer came out to check on him.
Minnesota manager Ron Gardenhire joined them on the mound shortly after, and Blackburn walked to the dugout.
Right-hander Anthony Swarzak was called into emergency duty.
Blackburn needed 49 pitches to get through just 1 1/3 innings before he left. A team spokesman says he will be re-evaluated on Monday.