The third baseman was placed on the 15-day disabled list with a broken right wrist Saturday and Danny Valencia recalled from Triple-A Pawtucket.
Pedro Ciriaco was in manager Bobby Valentine's lineup as the third baseman, hitting ninth. Valencia, acquired in a trade from Minnesota on Sunday, was expected to arrive at about the same time as Boston's scheduled 6:05 p.m. start against the Cleveland Indians.
Middlebrooks, hitting .288 with 15 homers and 54 RBI in 75 games, was hit by a pitch in the ninth inning Friday night.
He is the 25th Red Sox player to go on the disabled list in 29 separate stints this year.