Arenado also missed Monday's game with a sore left wrist. He was able to swing a bat earlier in the day and is available to pinch hit, if necessary, per MLB.com.
The twins and three other men are charged with assaulting Erik Hood at a sports facility in Phoenix. Hood told police he was accosted and assaulted by the five men. Hood sustained a broken nose, bruises and abrasions during the incident.
Markieff Morris has denied being part the group that attacked Hood, but acknowledged he was at the sports complex.
The first court date for the twins is May 7.
"Markieff and Marcus are good men and I am confident that when all the facts come out, their names will be cleared," their agent Leon Rose said in a statememt, according to ESPN.com.
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