Mayo was assessed a flagrant two for the play and ejected with 1:52 left in the first quarter of the Bucks 112-104 loss to the Pelicans.
Following the game, Mayo said of the play, "I reacted the wrong way and handled it for sure the wrong way ... "It's definitely the wrong way to conduct yourself. I've got to be better and I will be better."
Mayo will serve his suspension Saturday when the Bucks host the Washington Wizards.
Mayo, 26, is averaging 11.8 points and 2.2 assists in 49 games this season.