Barber caught an 8-yard pass from Jay Cutler, then ran for 2 yards on the next play on third-and-1 to pick up a first down just before the end of the first quarter. It wasn't clear exactly how he was hurt.
The team announced the injury early in the second quarter.
The Bears signed Barber to a two-year contract July 30 after he was released by Dallas when the lockout ended. Barber spent the last six seasons with the Cowboys, running for 4,358 yards and 47 touchdowns.