Bills punter Brian Moorman hit a 49-yard punt, which Buffalo's Ruvell Martin downed at the Redskins 4-yard line. Back judge Craig Burd, however, ruled it a touchback.
The only Bills player who entered the end zone was Brad Smith, who never touched the ball.
Fans began booing when the ball was placed at the 20 and they got louder when a replay was shown on the scoreboard.
Coach Chan Gailey challenged the play, which was reversed shortly after referee David Scott reviewed it.
The NFL is using replacement officials after locking out its regular officials, whose contract expired.