All three were injured in a 31-7 loss to Carolina on Sunday that eliminated the Bolts from playoff contention.
Mathews broke his left collarbone, Floyd sustained an ankle injury and Wells had ankle and toe injuries.
Mathews, who broke his right collarbone on his first carry of the exhibition season, played in 12 regular-season games. He was the team's leading rusher with 707 yards and a touchdown on 184 carries.
Floyd led the Chargers with 56 catches for 814 yards and five touchdowns. Wells spent time on the Chargers' roster early in the season and then returned on Dec. 5. He started the past two games at right tackle.