Markov will be out for six months, according to the team, which announced Wednesday that he had a successful procedure to repair a torn anterior cruciate ligament in his right knee.
The Russian All-Star has missed eight games since he was injured during the Canadiens' 6-3 loss in Pittsburgh on April 30.
Markov practiced Friday but did not accompany the team to Philadelphia, where Montreal was shut out in two straight losses to fall behind 2-0 in the Eastern Conference final. The Canadiens host Game 3 at Bell Centre on Thursday night.
Markov had four assists in eight playoff games. He had 34 points in 45 regular-season games.