Komisarek suffered the injury in Nashville on Thursday.
The 21-year-old center will be inserted into a lineup that is missing six regulars.
Colborne had 10 goals and 19 points in 13 games with the AHL's Marlies this season.
Also out for the Leafs are No. 1 goalie James Reimer (concussion-like symptoms) and forwards Clarke MacArthur (upper body), Mikhail Grabovski (lower body), Colby Armstrong (ankle sprain) and Matthew Lombardi (upper body).