Offensive Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Quarterback | J. Allen Josh Allen | D. Anderson Derek Anderson | |
| Running Back | L. McCoy LeSean McCoy | C. Ivory Chris Ivory | |
| Fullback | P. DiMarco Patrick DiMarco | — | — |
| Wide Receiver | K. Benjamin Kelvin Benjamin | Z. Jones Zay Jones | |
| Tight End | C. Clay Charles Clay | J. Croom Jason Croom | |
| Left Tackle | D. Dawkins Dion Dawkins | J. Sirles Jeremiah Sirles | — |
| Left Guard | V. Ducasse Vladimir Ducasse | W. Teller Wyatt Teller | — |
| Center | R. Bodine Russell Bodine | R. Groy Ryan Groy | — |
| Right Guard | J. Miller John Miller | — | — |
| Right Tackle | J. Mills Jordan Mills | C. McDermott Conor McDermott | — |
Defensive Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Left Defensive End | T. Murphy Trent Murphy | S. Lawson Shaq Lawson | — |
| Right Defensive Tackle | S. Lotulelei Star Lotulelei | H. Phillips Harrison Phillips | — |
| Left Defensive Tackle | K. Williams Kyle Williams | J. Phillips Jordan Phillips | — |
| Strongside Linebacker | L. Alexander Lorenzo Alexander | D. Lacey Deon Lacey | — |
| Middle Linebacker | T. Edmunds Tremaine Edmunds | J. Stanford Julian Stanford | — |
| Weakside Linebacker | M. Milano Matt Milano | — | — |
| Right Cornerback | L. Wallace Levi Wallace | R. Lewis Ryan Lewis | — |
| Left Cornerback | T. White Tre'Davious White | T. Johnson Taron Johnson | |
| Strong Safety | M. Hyde Micah Hyde | S. Neal Siran Neal | — |
| Free Safety | J. Poyer Jordan Poyer | R. Bush Rafael Bush | — |
Special Teams Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Punter | C. Schmidt Colton Schmidt | C. Bojorquez Corey Bojorquez | |
| Kicker | S. Hauschka Stephen Hauschka | — | — |
| Long Snapper | R. Ferguson Reid Ferguson | H. Phillips Harrison Phillips | — |
| Holder | C. Schmidt Colton Schmidt | — | — |
| Punt Returner | R. McCloud Ray-Ray McCloud | M. Murphy Marcus Murphy | |
| Kick Returner | M. Murphy Marcus Murphy | I. McKenzie Isaiah McKenzie | — |