Offense
| NO | Player | POS | HT | WT | DOB (AGE) | EXP | College |
|---|---|---|---|---|---|---|---|
| 10 | J. Garoppolo Jimmy Garoppolo | QB | 6-2 | 225 | 11/2/1991 (30) | 8 | Eastern Illinois |
| 5 | T. Lance Trey Lance | QB | 6-4 | 226 | 5/9/2000 (21) | R | North Dakota State |
| 49 | T. Cannon Trenton Cannon | RB | 5-11 | 185 | 7/23/1994 (27) | 3 | Virginia State |
| 23 | J. Hasty JaMycal Hasty | RB | 5-8 | 205 | 9/12/1996 (25) | 2 | Baylor |
| 25 | E. Mitchell Elijah Mitchell | RB | 5-10 | 200 | 5/2/1998 (23) | R | Louisiana |
| 28 | T. Sermon Trey Sermon | RB | 6-0 | 215 | 1/30/1999 (22) | R | Ohio State |
| 30 | J. Wilson Jeff Wilson | RB | 6-0 | 213 | 11/16/1995 (26) | 4 | North Texas |
| 44 | K. Juszczyk Kyle Juszczyk | FB | 6-1 | 235 | 4/23/1991 (30) | 9 | Harvard |
| 11 | B. Aiyuk Brandon Aiyuk | WR | 6-0 | 200 | 3/17/1998 (23) | R | Arizona State |
| 17 | T. Benjamin Travis Benjamin | WR | 5-10 | 175 | 12/29/1989 (31) | 9 | Miami (FL) |
| 15 | J. Jennings Jauan Jennings | WR | 6-3 | 212 | 7/10/1997 (24) | R | Tennessee |
| 19 | D. Samuel Deebo Samuel | WR | 6-0 | 215 | 1/15/1996 (25) | 3 | South Carolina |
| 81 | T. Sherfield Trent Sherfield | WR | 6-1 | 219 | 2/26/1996 (25) | 4 | Vanderbilt |
| 82 | R. Dwelley Ross Dwelley | TE | 6-5 | 235 | 1/26/1995 (26) | 4 | San Diego |
| 85 | G. Kittle George Kittle | TE | 6-4 | 250 | 10/9/1993 (28) | 5 | Iowa |
| 89 | C. Woerner Charlie Woerner | TE | 6-5 | 241 | 10/16/1997 (24) | R | Georgia |
| 64 | J. Brendel Jake Brendel | C | 6-4 | 299 | 9/10/1992 (29) | 3 | UCLA |
| 50 | A. Mack Alex Mack | C | 6-4 | 311 | 11/19/1985 (36) | 13 | California |
| 68 | C. McKivitz Colton McKivitz | OT | 6-6 | 301 | 8/9/1996 (25) | R | West Virginia |
| 76 | J. Moore Jaylon Moore | OT | 6-4 | 311 | 1/9/1998 (23) | R | Western Michigan |
| 71 | T. Williams Trent Williams | OT | 6-5 | 320 | 7/19/1988 (33) | 10 | Oklahoma |
| 65 | A. Banks Aaron Banks | OG | 6-5 | 325 | 9/3/1997 (24) | R | Notre Dame |
| 60 | D. Brunskill Daniel Brunskill | OG | 6-5 | 300 | 1/27/1994 (27) | 3 | San Diego State |
| 66 | T. Compton Tom Compton | OG | 6-6 | 315 | 5/10/1989 (32) | 9 | South Dakota |
| 75 | L. Tomlinson Laken Tomlinson | OG | 6-3 | 315 | 2/9/1992 (29) | 7 | Duke |
Defense
| NO | Player | POS | HT | WT | DOB (AGE) | EXP | College |
|---|---|---|---|---|---|---|---|
| 91 | A. Armstead Arik Armstead | DE | 6-7 | 290 | 11/15/1993 (28) | 7 | Oregon |
| 97 | N. Bosa Nick Bosa | DE | 6-4 | 266 | 10/23/1997 (24) | 3 | Ohio State |
| 56 | S. Ebukam Samson Ebukam | DE | 6-3 | 245 | 5/9/1995 (26) | 5 | Eastern Washington |
| 98 | A. Key Arden Key | DE | 6-5 | 240 | 5/3/1996 (25) | 4 | LSU |
| 92 | C. Omenihu Charles Omenihu | DE | 6-5 | 280 | 8/20/1997 (24) | 3 | Texas |
| 78 | J. Willis Jordan Willis | DE | 6-4 | 270 | 5/2/1995 (26) | 5 | Kansas State |
| 90 | K. Givens Kevin Givens | DT | 6-1 | 285 | 3/1/1997 (24) | 2 | Penn State |
| 96 | M. Hurst Maurice Hurst | DT | 6-2 | 291 | 5/9/1995 (26) | 4 | Michigan |
| 93 | D. Jones D.J. Jones | DT | 6-0 | 305 | 1/19/1995 (26) | 5 | Ole Miss |
| 95 | K. Street Kentavius Street | DT | 6-2 | 287 | 5/8/1996 (25) | 3 | NC State |
| 51 | A. Al-Shaair Azeez Al-Shaair | OLB | 6-2 | 228 | 8/4/1997 (24) | 3 | Florida Atlantic |
| 45 | D. Flannigan-Fowles Demetrius Flannigan-Fowles | OLB | 6-2 | 223 | 9/4/1996 (25) | 2 | Arizona |
| 57 | D. Greenlaw Dre Greenlaw | OLB | 6-0 | 230 | 5/25/1997 (24) | 3 | Arkansas |
| 36 | M. Harris Marcell Harris | OLB | 6-0 | 215 | 6/9/1994 (27) | 4 | Florida |
| 54 | F. Warner Fred Warner | MLB | 6-3 | 230 | 11/19/1996 (25) | 4 | BYU |
| 38 | D. Lenoir Deommodore Lenoir | CB | 5-10 | 200 | 10/6/1999 (22) | R | Oregon |
| 4 | E. Moseley Emmanuel Moseley | CB | 5-11 | 190 | 3/25/1996 (25) | 4 | Tennessee |
| 26 | J. Norman Josh Norman | CB | 6-0 | 200 | 12/15/1987 (33) | 10 | Coastal Carolina |
| 20 | A. Thomas Ambry Thomas | CB | 6-0 | 190 | 9/9/1999 (22) | R | Michigan |
| 27 | D. Johnson Dontae Johnson | DB | 6-2 | 200 | 12/1/1991 (30) | 8 | NC State |
| 24 | K. Williams K'Waun Williams | DB | 5-9 | 185 | 7/12/1991 (30) | 7 | Pittsburgh |
| 1 | J. Ward Jimmie Ward | FS | 5-11 | 195 | 7/18/1991 (30) | 8 | Northern Illinois |
| 29 | T. Hufanga Talanoa Hufanga | SS | 6-0 | 200 | 2/2/1999 (22) | R | USC |
| 3 | J. Tartt Jaquiski Tartt | SS | 6-1 | 215 | 2/12/1992 (29) | 7 | Samford |
Special Teams
| NO | Player | POS | HT | WT | DOB (AGE) | EXP | College |
|---|---|---|---|---|---|---|---|
| 9 | R. Gould Robbie Gould | K | 6-0 | 190 | 12/6/1982 (38) | 17 | Penn State |
| 18 | M. Wishnowsky Mitch Wishnowsky | P | 6-2 | 220 | 3/2/1992 (29) | 3 | Utah |
| 46 | T. Pepper Taybor Pepper | LS | 6-4 | 245 | 5/28/1994 (27) | 3 | Michigan State |