Offensive Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Quarterback | C. Beathard C.J. Beathard | — | |
| Running Back | J. Wilson Jeff Wilson | J. McKinnon Jerick McKinnon | |
| Fullback | K. Juszczyk Kyle Juszczyk | J. Hokit Josh Hokit | — |
| Wide Receiver | D. Samuel Deebo Samuel | B. Aiyuk Brandon Aiyuk | |
| Tight End | G. Kittle George Kittle | J. Reed Jordan Reed | |
| Left Tackle | J. Skule Justin Skule | — | — |
| Left Guard | L. Tomlinson Laken Tomlinson | — | — |
| Center | D. Brunskill Daniel Brunskill | H. Grasu Hroniss Grasu | — |
| Right Guard | T. Bergstrom Tony Bergstrom | C. McKivitz Colton McKivitz | — |
| Right Tackle | M. McGlinchey Mike McGlinchey | — | — |
Defensive Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Left Defensive End | A. Armstead Arik Armstead | — | |
| Right Defensive End | K. Hyder Kerry Hyder | D. Jordan Dion Jordan | |
| Left Defensive Tackle | J. Kinlaw Javon Kinlaw | K. Street Kentavius Street | |
| Right Defensive Tackle | D. Jones D.J. Jones | D. Daniels Darrion Daniels | |
| Strongside Linebacker | D. Greenlaw Dre Greenlaw | M. Nzeocha Mark Nzeocha | — |
| Middle Linebacker | F. Warner Fred Warner | A. Al-Shaair Azeez Al-Shaair | — |
| Weakside Linebacker | D. Flannigan-Fowles Demetrius Flannigan-Fowles | J. Walker Joe Walker | — |
| Right Cornerback | J. Verrett Jason Verrett | E. Moseley Emmanuel Moseley | |
| Left Cornerback | R. Sherman Richard Sherman | A. Witherspoon Ahkello Witherspoon | |
| Strong Safety | M. Harris Marcell Harris | K. Nacua Kai Nacua | |
| Free Safety | J. Ward Jimmie Ward | T. Moore Tarvarius Moore | — |
Special Teams Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Punter | M. Wishnowsky Mitch Wishnowsky | — | — |
| Kicker | R. Gould Robbie Gould | — | — |
| Holder | M. Wishnowsky Mitch Wishnowsky | — | — |
| Punt Returner | B. Aiyuk Brandon Aiyuk | T. Taylor Trent Taylor | |
| Kick Returner | J. McKinnon Jerick McKinnon | — | — |