Offensive Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Quarterback | N. Mullens Nick Mullens | C. Beathard C.J. Beathard | |
| Running Back | M. Breida Matt Breida | A. Morris Alfred Morris | |
| Fullback | K. Juszczyk Kyle Juszczyk | — | — |
| Wide Receiver | M. Goodwin Marquise Goodwin | P. Garcon Pierre Garcon | |
| Tight End | G. Kittle George Kittle | R. Dwelley Ross Dwelley | |
| Left Tackle | J. Staley Joe Staley | S. Coleman Shon Coleman | — |
| Left Guard | L. Tomlinson Laken Tomlinson | — | — |
| Center | W. Richburg Weston Richburg | E. Magnuson Erik Magnuson | — |
| Right Guard | M. Person Mike Person | J. Garnett Joshua Garnett | — |
| Right Tackle | M. McGlinchey Mike McGlinchey | G. Gilliam Garry Gilliam | — |
Defensive Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Left Defensive End | S. Thomas Solomon Thomas | C. Marsh Cassius Marsh | — |
| Right Defensive Tackle | D. Buckner DeForest Buckner | S. Day Sheldon Day | — |
| Left Defensive Tackle | E. Mitchell Earl Mitchell | D. Jones D.J. Jones | — |
| Strongside Linebacker | D. Watson Dekoda Watson | E. Lee Elijah Lee | — |
| Middle Linebacker | F. Warner Fred Warner | B. Coyle Brock Coyle | — |
| Weakside Linebacker | R. Foster Reuben Foster | M. Smith Malcolm Smith | |
| Right Cornerback | A. Witherspoon Ahkello Witherspoon | T. Moore Tarvarius Moore | |
| Left Cornerback | R. Sherman Richard Sherman | K. Williams K'Waun Williams | |
| Strong Safety | J. Tartt Jaquiski Tartt | A. Exum Antone Exum | |
| Free Safety | J. Ward Jimmie Ward | D. Reed D.J. Reed |
Special Teams Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Punter | B. Pinion Bradley Pinion | — | — |
| Kicker | R. Gould Robbie Gould | — | — |
| Long Snapper | K. Nelson Kyle Nelson | — | — |
| Holder | B. Pinion Bradley Pinion | — | — |
| Punt Returner | R. James Richie James | D. Pettis Dante Pettis | |
| Kick Returner | R. James Richie James | — | — |