Offensive Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Quarterback | M. Mariota Marcus Mariota | B. Gabbert Blaine Gabbert | — |
| Running Back | D. Henry Derrick Henry | D. Lewis Dion Lewis | |
| Wide Receiver | C. Davis Corey Davis | T. Sharpe Tajae Sharpe | |
| Tight End | J. Smith Jonnu Smith | L. Stocker Luke Stocker | |
| Left Tackle | T. Lewan Taylor Lewan | D. Kelly Dennis Kelly | |
| Left Guard | Q. Spain Quinton Spain | A. Stinnie Aaron Stinnie | — |
| Center | B. Jones Ben Jones | C. Levin Corey Levin | — |
| Right Guard | J. Kline Josh Kline | — | — |
| Right Tackle | J. Conklin Jack Conklin | — | — |
Defensive Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Left Defensive End | D. Jones DaQuan Jones | M. Dickerson Matt Dickerson | — |
| Nose Tackle | A. Johnson Austin Johnson | B. Logan Bennie Logan | |
| Right Defensive End | J. Casey Jurrell Casey | — | — |
| Left Inside Linebacker | W. Woodyard Wesley Woodyard | J. Brown Jayon Brown | |
| Right Inside Linebacker | R. Evans Rashaan Evans | W. Compton Will Compton | — |
| Strongside Linebacker | B. Orakpo Brian Orakpo | K. Correa Kamalei Correa | — |
| Weakside Linebacker | D. Morgan Derrick Morgan | H. Landry Harold Landry | |
| Right Cornerback | L. Ryan Logan Ryan | A. Jackson Adoree' Jackson | — |
| Left Cornerback | M. Butler Malcolm Butler | L. Sims LeShaun Sims | |
| Strong Safety | K. Vaccaro Kenny Vaccaro | K. Lewis Kendrick Lewis | — |
| Free Safety | K. Byard Kevin Byard | D. Cruikshank Dane Cruikshank |
Special Teams Positions
| POS | Starter | Second | Third+ |
|---|---|---|---|
| Punter | B. Kern Brett Kern | — | — |
| Kicker | R. Succop Ryan Succop | — | — |
| Long Snapper | B. Brinkley Beau Brinkley | — | — |
| Holder | B. Kern Brett Kern | B. Gabbert Blaine Gabbert | — |
| Punt Returner | A. Jackson Adoree' Jackson | — | — |
| Kick Returner | D. Jennings Darius Jennings | A. Jackson Adoree' Jackson |